4 Kalite Viris Respiratwa
Non pwodwi
HWTS-RT099- Twous Deteksyon Asid Nikleyik 4 Kalite Viris Respiratwa (PCR Fliyoresans)-Sistèm PCR an Tan Reyèl NED-ABI 7500/Sistèm PCR rapid an tan reyèl ABI 7500/QuantStudio®5 Sistèm PCR an tan reyèl
Twous deteksyon asid nikleyik HWTS-RT158-4 Kalite viris respiratwa (PCR fliyoresans)内参Quasar 705
Epidemyoloji
Maladi Viris Kowonaviris 2019 la, ke yo rele "COVID-19", refere a nemoni ki koze pa2019-nCoVenfeksyon.2019-nCoVse yon kowonaviris ki fè pati genus β a. COVID-19 se yon maladi enfeksyon respiratwa egi, epi popilasyon an jeneralman sansib. Kounye a, sous enfeksyon an se sitou pasyan ki enfekte pa2019-nCoV, epi moun ki enfekte san sentòm yo kapab tou vin sous enfeksyon an. Baze sou ankèt epidemyolojik aktyèl la, peryòd enkubasyon an se 1-14 jou, sitou 3-7 jou. Lafyèv, tous sèk ak fatig se manifestasyon prensipal yo. Gen kèk pasyan ki te gen sentòmtankoukonjesyon nan nen, nen k ap koule, gòj fè mal, myalji ak dyare, elatriye.
Chanèl
| FAM | 2019-nKowonaviris |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Kontwòl Entèn |
Paramèt teknik
| Depo | -18℃ |
| Dire lavi etajè | 9 mwa |
| Kalite echantiyon | Prelèvman orofarenjyen |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300 Kopi/mLViris grip A/Viris grip B/Viris respiratwa senksyal: 500 Kopi/mL |
| Espesifikite | a) Rezilta kwa-reyaktivite yo montre pa gen okenn kwa-reyaktivite ant twous la ak kowonaviris imen SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, viris parainfluenza tip 1, 2, 3, rinovirus A, B, C, chlamydia pneumoniae, metapneumovirus imen, enterovirus A, B, C, D, viris poumon imen, viris epstein-barr, viris lawoujòl, viris sitomegalo imen, rotavirus, norovirus, viris parotit, viris varicella-zostèr, lejyonèl, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, aspergillus lafimen, candida albicans, candida glabrata, pneumocystis jiroveci ak tibebe ki fèk fèt. kriptokok ak asid nikleyik jenomik imen. b) Kapasite anti-entèferans: chwazi mucin (60mg/mL), 10% (v/v) nan san ak fenilefrin (2mg/mL), oksimetazolin (2mg/mL), klori sodyòm (ki gen ladan préservatif) (20 mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alfa interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azitromisin (1mg/mL), seftriyaksòn (40μg/mL), meropenèm (200mg/mL), levofloksasin (10μg/mL) ak tobramisin (0.6mg/mL) pou tès entèferans, epi rezilta yo montre ke sibstans ki entèfere ak konsantrasyon yo mansyone pi wo a pa gen okenn reyaksyon entèferans ak rezilta tès patojèn yo. |
| Enstriman Aplikab yo | Sistèm PCR an tan reyèl Applied Biosystems 7500Sistèm PCR rapid an tan reyèl Applied Biosystems 7500 Sistèm PCR an tan reyèl QuantStudio®5 |
Koule Travay
Opsyon 1.
Twous ADN/ARN viral Macro & Micro-Test (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ak Ekstraktè Asid Nikleyik Otomatik Macro & Micro-Test (HWTS-3006) ki te pwodui pa Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Volim echantiyon ki te ekstrè a se 200μL, epi volim elisyon rekòmande a se 80μL.
Opsyon 2.
Twous Mini RNA Viral QIAamp (52904) pwodui pa QIAGEN oubyen Twous Ekstraksyon oubyen Pirifikasyon Asid Nukleik (YDP315-R) pwodui pa Tiangen Biotech (Beijing) Co., Ltd. Volim echantiyon ki te ekstrè a se 140μL, epi volim elisyon rekòmande a se 60μL.
