4 Kalite Viris Respiratwa
Non pwodwi
HWTS-RT099- 4 Kalite Viris Respiratwa Twous Deteksyon Asid Nukleik (PCR Fluoresans)
Epidemyoloji
Maladi Corona Viris 2019, ki rele "COVID-19", refere a nemoni ki te koze pa2019-nCoVenfeksyon.2019-nCoVse yon coronavirus ki fè pati genus β.COVID-19 se yon maladi enfektye respiratwa egi, epi popilasyon an jeneralman sansib.Kounye a, sous enfeksyon se sitou pasyan ki enfekte pa2019-nCoV, ak moun ki asymptomatik ki enfekte ka vin sous enfeksyon tou.Dapre envestigasyon epidemyolojik aktyèl la, peryòd enkubasyon an se 1-14 jou, sitou 3-7 jou.Lafyèv, tous sèk ak fatig se manifestasyon prensipal yo.Kèk pasyan te gen sentòms tankoukonjesyon nan nen, nen k ap koule, gòj fè mal, myalji ak dyare, elatriye.
Chèn
| FAM | 2019-nCoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Kontwòl Entèn |
Paramèt teknik
| Depo | -18℃ |
| Etajè-lavi | 9 mwa |
| Kalite echantiyon | Prelèvman oropharyngeal |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Kopi/mLViris Grip A/Viris Grip B/Viris respiratwa syncytial: 500Kopi/mL |
| Espesifik | a) Rezilta kwa-reaktivite yo montre ke pa gen okenn reyaksyon kwaze ant twous la ak kowonaviris imen SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, viris parainfluenza tip 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, metapneumovirus imen, enterovirus A, B, C, D, viris pulmonè imen, viris epstein-barr, viris lawoujòl, viris cytomegalo imen, rotaviris, norovirus, viris parotitis, varisèl-zoster. viris, legionella, bordetella koklich, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tibèkiloz, aspergillus lafimen, candida albicans, candida glabrata, candida glabrata ak nouvo asid kriptonklè ak nouvo gen moun kryptococcusus. b) Kapasite anti-entèferans: chwazi mucin (60mg/mL), 10% (v/v) nan san ak phenylephrine (2mg/mL), oxymetazoline (2mg/mL), klori sodyòm (ki gen ladan préservatifs) (20 mg/mL). ), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL). ), idroklorid histamin (5mg/mL), entèferon alfa (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL). mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azitromisin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) ak tobramycin (0.6mg/mL). mL) pou tès entèferans, ak rezilta yo montre ke sibstans entèfere ak konsantrasyon mansyone pi wo a pa gen okenn reyaksyon entèferans nan rezilta tès patojèn yo. |
| Enstriman ki aplikab | Applied Biosystems 7500 an tan reyèl sistèm PCR Applied Biosystems 7500 rapid an tan reyèl sistèm PCR QuantStudio®5 Sistèm PCR an tan reyèl |
Travay koule
Opsyon 1.
Macro & Micro-Test Viral DNA/RNA Twous (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ak Macro & Micro-Test Otomatik Ekstraktè Asid Nukleik (HWTS-3006) ki te pwodwi pa Jiangsu Macro & Micro -Test Med-Tech Co, Ltd volim echantiyon ekstrè se 200μL, ak volim elisyon rekòmande a se 80μL.
Opsyon 2.
QIAamp Viral RNA Mini Twous (52904) ki te pwodwi pa QIAGEN oswa Ekstraksyon Asid Nukleik oswa Pirifikasyon Twous (YDP315-R) ki te pwodwi pa Tiangen Biotech (Beijing) Co, Ltd. Volim echantiyon ekstrè se 140μL, ak volim elisyon rekòmande a se 60μL.
