4 kalite viris respiratwa
Non pwodwi
HWTS-RT099- 4 Kalite Viris Respiratwa Twous Deteksyon Asid Nukleik (Fluoresans PCR)
Epidemyoloji
Maladi Viris Corona 2019, refere yo kòm "Covid-19", refere a nemoni a ki te koze pa2019-nCoVenfeksyon.2019-nCoVse yon coronavirus ki fè pati β genus la. COVID-19 se yon maladi respiratwa respiratwa egi, ak popilasyon an se jeneralman sansib. Kounye a, se sous enfeksyon an sitou pasyan ki enfekte pa2019-nCoV, ak senptomatik ki enfekte moun ka tou vin sous la nan enfeksyon. Baze sou envestigasyon aktyèl la epidemyoloji, peryòd la enkubasyon se 1-14 jou, sitou 3-7 jou. Lafyèv, tous sèk ak fatig yo se manifestasyon prensipal yo. Yon kèk pasyan te gen sentòms tankouKonjesyon nan nen, nen k ap koule, gòj fè mal, myalgie ak dyare, elatriye.
Kanal
| Fam | 2019-nCOV |
| Vic (Egzagòn) | RSV |
| Cy5 | Ifv a |
| Rox | IFV B |
| Ned | Kontwòl Entèn |
Paramèt teknik
| Stokaj | -18 ℃ |
| Etajè-lavi | 9 mwa |
| Kalite echantiyon | Orofaryngeal prelèvman |
| Ct | ≤ 38 |
| LOD | 2019-NCOV: 300copies/mlViris grip A viris/grip B viris/respiratwa viris syncytial: 500copies/ml |
| Espesifik | a) Rezilta kwa-reyaktivite montre ke pa gen okenn reyaksyon kwa ant twous la ak moun coronavirus Sarsr-CoV, MERSR-CoV, HCOV-OC43, HCOV-229E, HCOV-HKU1, HCOV-NL63, Parainfluenza Viris Kalite 1, 2, 3, Rhinovirus A, B, C, Chlamydia pneumoniae, moun metapneumovirus, enterovirus A, B, C, D, human pulmonary virus, epstein-barr virus, measles virus, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, strèptokok pneumoniae, streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, lafimen Aspergillus, Candida albicans, Candida glabrata, Pneumocystis Jiroveci ak Cryptococcus ki fèk fèt ak Imèn Numleic Nurveci. b) Anti-entèferans kapasite: Chwazi mucin (60mg/ml), 10% (v/v) nan san ak fenilfrin (2mg/ml), oxymetazoline (2mg/mL), klori sodyòm (ki gen ladan préservatifs) (20 mg/ml ), beclomethasone (20mg/ml), dexamethasone (20mg/ml), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL ), Zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir(500mg/mL), ritonavir(60mg/mL), mupirocin (20mg/mL), azithromycin ( 1mg/ml), ceftriaxone (40μg/ml), meropenem (200mg/ml), levofloxacin (10μg/ml) ak tobramycin (0.6mg/mL) pou tès entèferans, ak rezilta yo montre ke entèfere sibstans ki sou ak konsantrasyon mansyone pi wo a pa gen okenn reyaksyon entèferans nan rezilta tès la rezilta tès yo nan ajan patojèn. |
| Enstriman ki aplikab yo | Aplike Biosystems 7500 an tan reyèl sistèm PCR Aplike Biosystems 7500 vit sistèm PCR an tan reyèl QuantStudio®5 Sistèm PCR an tan reyèl |
Travay koule
Opsyon 1.
Macro & mikwo-tès viral ADN/RNA twous (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ak Macro & Micro-tès otomatik asid nwayo (HWTS-3006) pwodwi pa Jiangsu Macro & Micro -Test Med-Tech Co., Ltd. volim nan echantiyon extrait se 200μl, ak la Rekòmande volim elution se 80μl.
Opsyon 2.
QIAAMP viral RNA Mini Twous (52904) ki te pwodwi pa Qiagen oswa ekstraksyon asid nikleyik oswa twous pou pirifye (YDP315-R) ki te pwodwi pa Tiangen Biotech (Beijing) co, Ltd. Volim nan echantiyon extrait se 140μl, ak volim nan elution rekòmande se 60μl.
