Grip A viris/ grip B viris
Non pwodwi
HWTS-RT174-enfluenza A viris/ grip B viris Nikleyik Deteksyon Deteksyon Twous (fluoresans PCR)
Epidemyoloji
Baze sou diferans ki genyen antigenic ant jèn NP ak M jèn, viris grip ka divize an kat kalite: viris grip A (IFV A), viris grip B (IFV B), viris grip C (IFV C) ak viris grip D (IFV D)[1]. Viris grip A gen anpil gen tout pouvwa a ak serotip konplèks, epi yo ka jwenn kapasite nan gaye nan tout gen tout pouvwa a nan recombination jenetik ak mitasyon adaptasyon. Moun manke iminite ki dire lontan nan viris grip la, se konsa moun ki gen tout laj yo jeneralman sansib. Viris grip A se patojèn prensipal la ki lakòz pandemi grip la[2]. Viris grip B se sitou répandus nan yon ti zòn ak kounye a pa gen okenn subtypes. Moun prensipal yo ki lakòz enfeksyon imen yo se filaj la B/Yamagata oswa filiation la B/Victoria. Pami ka yo konfime nan grip nan 15 peyi nan rejyon Pasifik la Azi chak mwa, pousantaj la konfime nan viris grip B se 0-92%[3]. Kontrèman ak viris grip A, gwoup espesifik tankou timoun yo ak granmoun aje yo sansib a viris grip B epi yo gen tandans fè konplikasyon, ki enpoze yon pi gwo fado sou sosyete a pase viris grip A[4].
Kanal
| Fam | MP asid nikleyik |
| Rox | Kontwòl Entèn |
Paramèt teknik
| Stokaj | ≤-18 ℃ |
| Etajè-lavi | 12 mwa |
| Kalite echantiyon | Echantiyon prelèvman orofarinyen |
| Ct | Grip a, grip bCT≤35 |
| CV | <5.0% |
| LOD | Grip a ak grip byo tout 200copies/ml |
| Espesifik | Cross-reactivity: There is no cross reaction between the kit and Bocavirus, rhinovirus, cytomegalovirus, respiratory syncytial virus, parainfluenza virus, Epstein-Barr virus, herpes simplex virus, Varicella-zoster virus, mumps virus, enterovirus, measles virus, human metapneumovirus , adenovirus, imen coronavirus, novel coronavirus, SARS coronavirus, MERS coronavirus, rotavirus, norovirus, Chlamydia pneumoniae, Mycoplasma pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumocystis carinii, Haemophilus influenzae, Bordetella pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, Neisseria gonorrhoeae, Candida albicans, Candida glabrata, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrhalis, Lactobacillus, Corynebacterium, ak ADN jenomik imen. Tès entèferans: Chwazi mucin (60 mg/ml), san moun (50%), fenilfrin (2mg/ml), oxymetazoline (2mg/ml), klori sodyòm (20mg/ml) ak 5% konsèvasyon, beclomethasone (20mg/ml ), dexamethasone (20mg/ml), flunisolide (20μg/ml), triamcinolone acetonide (2mg/ml), budesonide (1mg/mL), mometasone (2mg/ml), fluticasone (2mg/ml), histamin hydrochloride (5mg/ml), benzocaine (10%), 10%) ( (10%), zanamivir (20mg/ml), Peramivir (1mg/ml), mupirocin (20mg/ml), tobramycin (0.6mg/ml), oseltamivir (60ng/ml), ribavirin (10mg/L) pou tès entèferans, ak rezilta yo montre ke sibstans ki sou yo entèfere nan pi wo a Konsantrasyon pa entèfere ak deteksyon an nan twous la. |
| Enstriman ki aplikab yo | SLAN-96P Sistèm PCR an tan reyèl Aplike Biosystems 7500 an tan reyèl sistèm PCR QuantStudio®5 Sistèm PCR an tan reyèl Linegene 9600 plis sistèm deteksyon PCR an tan reyèl MA-6000 an tan reyèl quantitative tèmik Cycler Biorad CFX96 an tan reyèl sistèm PCR Biorad cfx opus 96 an tan reyèl sistèm PCR |
Travay koule
Macro & mikwo-tès viral ADN/RNA twous (HWTS-3017) (ki ka itilize ak macro & mikwo-tès otomatik asid nikleyik (HWTS-3006C, HWTS-3006B)) pa Jiangsu Macro & mikwo-tès Med-Tech-Tech-Tchesse Co, Ltd.yo rekòmande pou ekstraksyon echantiyon an aketap ki vin apre yo ta dwekonduited nan dapre strik ak IFU lanan twous la.
