Viris Grip A / Viris Grip B
Non pwodwi
HWTS-RT174-Twous Deteksyon Asid Nikleyik Viris Grip A/Viris Grip B (PCR Fliyoresans)
Epidemyoloji
Baze sou diferans antijenik ki genyen ant jèn NP ak jèn M, viris grip yo ka divize an kat kalite: viris grip A (IFV A), viris grip B (IFV B), viris grip C (IFV C) ak viris grip D (IFV D).[1]Viris grip A a gen anpil lame ak serotip konplèks, epi li ka gen kapasite pou l gaye nan mitan lame yo atravè rekombinasyon jenetik ak mitasyon adaptatif. Moun pa gen iminite dirab kont viris grip A a, kidonk moun tout laj jeneralman sansib. Viris grip A a se prensipal patojèn ki lakòz pandemi grip yo.[2]Viris grip B a sitou répandu nan yon ti zòn epi kounye a li pa gen okenn soutip. Prensipal ka grip ki lakòz enfeksyon moun yo se liy B/Yamagata a oswa liy B/Victoria a. Pami ka grip konfime yo nan 15 peyi nan rejyon Azi Pasifik la chak mwa, to viris grip B konfime a se 0-92%.[3]Kontrèman ak viris grip A a, gen gwoup espesifik tankou timoun ak granmoun aje ki vilnerab a viris grip B a epi ki gen tandans pou yo gen konplikasyon, sa ki mete yon pi gwo chay sou sosyete a pase viris grip A a.[4].
Chanèl
| FAM | Asid nikleyik MP |
| ROX | Kontwòl Entèn |
Paramèt teknik
| Depo | ≤-18℃ |
| Dire lavi etajè | 12 mwa |
| Kalite echantiyon | Echantiyon prelèvman orofarenjyen |
| Ct | Grip A, Grip BCt≤35 |
| CV | <5.0% |
| LoD | Grip A ak Grip Btout se 200 Kopi/mL |
| Espesifikite | Reyaktivite kwaze: Pa gen okenn reyaksyon kwaze ant twous la ak Bocavirus, rinovirus, sitomegalovirus, viris respiratwa senksyal, viris parainfluenza, viris Epstein-Barr, viris èpès senp, viris Varicella-zostèr, viris oreyon, enterovirus, viris lawoujòl, metapneumovirus imen, adenovirus, kowonaviris imen, nouvo kowonaviris, kowonaviris SARS, kowonaviris MERS, rotavirus, norovirus, Chlamydia pneumoniae, Mycoplasma pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumocystis carinii, Haemophilus influenzae, Bordetella pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, Neisseria gonorrhoeae, Candida albicans, Candida glabrata, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrhalis, Lactobacillus, Corynebacterium, ak ADN jenomik imen. Tès entèferans: Chwazi mucin (60 mg/mL), san moun (50%), fenilefrin (2mg/mL), oksimetazolin (2mg/mL), klori sodyòm (20mg/mL) ak 5% konsèvatif, beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzokayin (10%), mantòl (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir (60ng/mL), ribavirin (10mg/L) pou entèferans. tès yo, epi rezilta yo montre ke sibstans ki entèfere yo nan konsantrasyon ki anwo yo pa entèfere ak deteksyon twous la. |
| Enstriman Aplikab yo | Sistèm PCR an tan reyèl SLAN-96P Sistèm PCR an tan reyèl Applied Biosystems 7500 QuantStudio®5 Sistèm PCR an tan reyèl Sistèm Deteksyon PCR an Tan Reyèl LineGene 9600 Plus MA-6000 Siklis tèmik kantitatif an tan reyèl Sistèm PCR an tan reyèl BioRad CFX96 la Sistèm PCR an tan reyèl BioRad CFX Opus 96 |
Koule Travay
Twous ADN/ARN viral Macro & Micro-Test (HWTS-3017) (ki ka itilize avèk Ekstraktè Asid Nikleyik Otomatik Macro & Micro-Test (HWTS-3006C, HWTS-3006B)) pa Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.yo rekòmande pou ekstraksyon echantiyon an ak laetap ki vin apre yo ta dwekonduifèt an akò strik avèk IFU anan Twous la.
