Patojèn respiratwa konbine
Non pwodwi
HWTS-RT050-Sis kalite twous deteksyon asid nikleik patojèn respiratwa(PCR fluoresans)
Epidemyoloji
Grip, souvan ke yo rekonèt kòm 'grip', se yon maladi enfektye respiratwa egi ki te koze pa viris grip, ki trè enfektye epi li se sitou transmèt pa touse ak etènye.
Viris respiratwa syncytial (RSV) se yon viris RNA, ki fè pati fanmi paramyxoviridae.
Adenoviris imen (HAdV) se yon viris ADN doub ki pa gen anvlòp.Yo te jwenn omwen 90 jenotip, ki ka divize an 7 subgenera AG.
Rinoviris imen (HRV) se yon manm fanmi Picornaviridae ak genus Enterovirus.
Mycoplasma pneumoniae (MP) se yon mikwo-òganis patojèn ki se ant bakteri ak viris nan gwosè.
Chèn
| Chèn | PCR-Mix A | PCR-Mix B |
| FAM Channel | IFV A | HAdV |
| VIC/HEX Channel | HRV | IFV B |
| CY5 Chèn | RSV | MP |
| Chèn ROX | Kontwòl Entèn | Kontwòl Entèn |
Paramèt teknik
| Depo | -18℃ |
| Etajè-lavi | 12 mwa |
| Kalite echantiyon | Prelèvman oropharyngeal |
| Ct | ≤35 |
| LoD | 500Kopi/mL |
| Espesifik | 1.Rezilta tès kwa-reaktivite yo te montre ke pa te gen okenn reyaksyon kwaze ant twous lan ak kowonaviris imen SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, Parainfluenza viris kalite 1, 2, ak 3, Chlamydia pneumoniae, metapneumovirus imen, Enterovirus A, B, C, D, viris Epstein-Barr, viris lawoujòl, cytomegalovirus imen, Rotaviris, Norovirus, Viris Malmouton, Viris Varicella-zoster, Legionella, Bordetella koklich, Haemophilus influenzae, Staphylococcus. aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata, Pneumocystis jiroveci, Cryptococcus neoformans ak asid nikleyik jenomik imen. 2.Kapasite anti-entèferans: Mucin (60mg/mL), 10% (v/v) san moun, phenylephrine (2mg/mL), oxymetazoline (2mg/mL), klori sodyòm (ak préservatifs) (20mg/mL), beclomethasone ( 20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), Fluticasone (2mg/mL), histamin idroklorid (5mg/mL), alfa-interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azitromisin (1mg/mL), cefprozil (40μg/mL), Meropenem (200mg/mL), levofloxacin (10μg/mL), ak tobramycin (0.6mg/mL) yo te chwazi pou tès la entèferans, ak rezilta yo te montre ke sibstans yo entèfere nan konsantrasyon ki anwo yo pa te gen okenn reyaksyon entèferans nan rezilta tès yo nan patojèn. |
| Enstriman ki aplikab | Applied Biosystems 7500 an tan reyèl sistèm PCR Applied Biosystems 7500 rapid an tan reyèl sistèm PCR QuantStudio®5 Sistèm PCR an tan reyèl SLAN-96P an tan reyèl sistèm PCR LightCycler®480 an tan reyèl sistèm PCR LineGene 9600 Plus an tan reyèl sistèm deteksyon PCR MA-6000 an tan reyèl Quantitative Thermal Cycler BioRad CFX96 an tan reyèl sistèm PCR, BioRad CFX Opus 96 an tan reyèl sistèm PCR |
Solisyon PCR total
