Uit kalite viris respiratwa
Non pwodwi
Hwts-rt184-Uit kalite viris respiratwa twous deteksyon asid nikleyik (fluoresans PCR)
Epidemyoloji
Enfeksyon nan aparèy respiratwa se kalite ki pi komen nan maladi moun, ki ka rive nan nenpòt ki sèks, laj ak rejyon an, e se youn nan kòz ki pi enpòtan nan morbidite ak lanmò nan mond lan[1]. Klinikman komen patojèn respiratwa gen ladan viris grip A (IFV A), viris grip B (IFV B), respiratwa viris syncytial, adenovirus, metapneumovirus imen, rinovirus, viris parainfluenza (I/II/III) ak mycoplasma pneumoniae, elatriye.[2,3]. Sentòm yo nan klinik ak siy ki te koze pa enfeksyon nan aparèy respiratwa yo relativman menm jan an, men enfeksyon an ki te koze pa ajan patojèn diferan gen metòd tretman diferan, efè guérison ak kou nan maladi[4,5]. Kounye a, metòd prensipal yo nan deteksyon laboratwa nan ajan patojèn respiratwa yo enkli: izolasyon viris, deteksyon antigen ak deteksyon asid nikleyik. Twous sa a detekte epi idantifye asid nikleyik viral espesifik nan moun ki gen siy ak sentòm enfeksyon respiratwa, an konjonksyon avèk lòt rezilta klinik ak laboratwa pou ede nan dyagnostik enfeksyon viral respiratwa.
Paramèt teknik
| Stokaj | 2-8 ℃ |
| Etajè-lavi | 12 mwa |
| Kalite echantiyon | Prelèvman orofarin; Nasopharyngeal prelèvman |
| Ct | IFV A, IFVB, RSV, ADV, HMPV, RHV, PIV, MP CT≤35 |
| CV | <5.0% |
| LOD | 200 kopi/ml |
| Espesifik | Lakwa reyaksyon: Pa gen okenn reyaksyon kwa ant twous la ak viris Boca, cytomegalovirus, Epstein-Barr viris, èpès viris senp, varisèl zoster viris, viris malmouton, enterovirus, viris lawouj, rolonavirus, sars coronavirus, mers coronavirus, RORONAVIRUS, SARS CORONAVIRUS, MERS CORONAVIRS, RORONAVIRUS, SARS , Chlamydia pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, Candida glabra, Aspergillus Fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrh, Lactobacillus, Corynebacterium, ADN jenomik moun. Tès entèferans: Chwazi mucin (60mg/mL), san moun (50%), Benephrin (2mg/ml), hydroxymethazoline (2mg/ml) 2mg/ml), klori sodyòm ak 5% konsèvasyon (20mg/ml), beclomethasone ( 20mg/ml), dexamethasone (20mg/ml), fluniacetone (20μg/mL), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), Zanamivir (20mg/ml), peramivir (1mg/ml), mupirocin (20mg/ml), tobramycin (0.6mg/ml), oseltamivir (60ng/ml), ribavirin (10mg/L), rezilta yo te montre ke sibstans yo entèfere nan concentra ki anwo a |
| Enstriman ki aplikab yo | Aplikab pou Kalite I Reyaktif Deteksyon: Aplike Biosystems 7500 Sistèm PCR an tan reyèl, QuantStudio®5 Sistèm PCR an tan reyèl, SLAN-96P Sistèm PCR an tan reyèl (Hongshi Medikal Teknoloji co, Ltd), Linegene 9600 plis sistèm deteksyon PCR an tan reyèl (FQD-96A, Hangzhou Bioer Teknoloji), MA-6000 reyèl- Tan quantitative tèmik Cycler (Suzhou Molarray co, Ltd), Biorad CFX96 an tan reyèl PCR Sistèm, Biorad CFX Opus 96 an tan reyèl sistèm PCR. Aplikab pou Kalite II deteksyon reyaktif: Eudemontm AIO800 (HWTS-EQ007) pa Jiangsu Macro & Micro-Tès Med-Tech co, Ltd. |
Travay koule
Macro & mikwo-tès viral ADN/RNA twous (HWTS-3017) (ki ka itilize ak macro & mikwo-tès otomatik asid nikleyik èkstraksyon (HWTS-3006C, HWTS-3006B)), ak Macro & Micro-tès viral ADN/ Twous RNA (HWTS-3017-8) (ki ka itilize ak eudemonTM AIO800 (HWTS-EQ007)) pa Jiangsu Macro & mikwo-tès Med-Tech co, Ltd.
Volim nan echantiyon extrait se 200μL ak volim nan elution rekòmande se 150μl.
